ygaK [2019-09-05 12:15:59]

uncharacterized FAD-linked oxidoreductase

locus: BSU_08800

pI: 7.12

mw: 40.96 kDa

protein length: 451 aa

gene length: 1356 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 957,705 959,060

The protein

Protein family

• oxygen-dependent FAD-linked oxidoreductase family (with [protein|23F2385D14DDD5C400DC897E0F0FC814ACFC36B8|YitY] and [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|CotQ], according to UniProt)

Paralogous protein(s)

• [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|CotQ]:

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|3W8W] (from ''Streptomyces maritimus'', 33%)

Expression and Regulation

Operons

• genes: [gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]
  description: [Pubmed|22383849]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-A246 (ygaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/246 NBRP B. subtilis, Japan]
• BKE08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC, downstream forward: _UP4_TAAAAAAATGAAGATGGAGC
• BKK08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC, downstream forward: _UP4_TAAAAAAATGAAGATGGAGC

References

• 22383849,27766092